TFDF Perfusion Boosts AAV and Lentiviral Vector Yields Up to 80-Fold

Published on Repligen Scientific Poster (Sept 2022)

To meet growing demand in gene therapy, Repligen scientists applied tangential flow depth filtration (TFDF) to intensify viral vector manufacturing. By integrating perfusion before and after transfection, they achieved 3× higher viable cell density and up to 30× greater per-cell productivity. Compared to batch culture, the TFDF process increased total AAV8 yields by ~10-fold and lentivirus yields by more than 80-fold, offering a powerful route toward continuous, scalable vector production.

Read the full poster from Repligen:

👉 KrosFlo TFDF-based intensified viral vector production (PDF)

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